Human HIV-Gp41 enzyme-linked immunosorbent assay (ELISA) kit instruction manual

This reagent is for research use only: This kit is used to determine HIV-Gp41 in human serum, plasma and related fluid samples. Experimental principle: This kit uses double antibody sandwich enzyme-linked immunosorbent assay (ELISA) to determine human HIV-Gp41 in the specimen. The microplate is coated with purified HIV-Gp41 antibody to prepare a solid phase antibody, which can be combined with human HIV-Gp41 in the sample, washed to remove unbound antigen and other components, and then HRP-labeled HIV-Gp41 antibody. The antibody-antigen-enzyme-labeled antibody complex was formed by binding, and after thorough washing, the substrate TMB was added for color development. TMB is converted to blue under the catalysis of HRP enzyme and converted to the final yellow color by the action of an acid. The absorbance (OD value) was measured at a wavelength of 450 nm using a microplate reader, and compared with the CUTOFF value to determine the presence or absence of human HIV-Gp41 in the specimen. Kit composition: Kit composition 48-well configuration 96-well configuration Storage instructions 1 part 1 part sealing film 2 pieces (48) 2 pieces (96) Sealed bag 1 1 enzyme label coated plate 1 × 48 1 × 96 2 -8 °C preservation negative control 0.5ml × 1 bottle 0.5ml × 1 bottle 2-8 ° C Preservation positive control 0.5ml × 1 bottle 0.5ml × 1 bottle 2-8 ° C Preservation enzyme standard reagent 3 ml × 1 bottle 6 ml × 1 Bottle 2-8 °C Preservation Sample Diluent 3 ml × 1 bottle 6 ml × 1 bottle 2-8 ° C Preservation developer A solution 3 ml × 1 bottle 6 ml × 1 bottle 2-8 ° C Preservation developer B solution 3 Ml × 1 bottle 6 ml × 1 bottle 2-8 ° C Preservation solution 3 ml × 1 bottle 6 ml × 1 bottle 2-8 ° C Preservation concentrated washing solution (20ml × 20 times) × 1 bottle (20ml × 30 times) × 1 bottle 2-8 ° C preservation

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