Human anti-human immunodeficiency virus antibody (HIV) enzyme-linked immunosorbent assay (ELISA) kit instruction manual

Human anti-human immunodeficiency virus antibody (HIV) enzyme-linked immunosorbent assay (ELISA) kit Instructions for use This kit is for research use only: This kit is for the detection of human serum, anti-human immunodeficiency virus antibody (HIV) antibodies in plasma Level. Experimental principle: This kit uses double antigen sandwich enzyme-linked immunosorbent assay (ELISA) to determine human anti-human immunodeficiency virus antibody (HIV) in the specimen. The microporous plate is coated with the purified antigen to prepare a solid phase antigen, which can be combined with the antibody in the sample, washed to remove unbound antigen and other components, and then combined with the HRP-labeled antigen to form an antigen-antibody-enzyme label. The antigen complex was thoroughly washed and added to the substrate TMB for color development. TMB is converted to blue under the catalysis of HRP enzyme and converted to the final yellow color by the action of an acid. The absorbance (OD value) was measured at 450 nm using a microplate reader, and compared with the CUTOFF value to determine the presence or absence of human anti-human immunodeficiency virus antibody (HIV) in the specimen.

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