This ELISA kit is used for the determination of interleukin 8 (IL-8) in chicken serum, plasma and related liquid samples. This kit uses the double antibody sandwich method to determine the level of chicken interleukin 8 (IL-8) in the specimen. With purified chicken white Interleukin 8 (IL-8) antibody is coated on the microwell plate to make a solid-phase antibody, and then interleukin is added to the monoclonal antibody-coated microwells in turn Interleukin 8 (IL-8), then combined with HRP-labeled interleukin 8 (IL-8) antibody to form antibody-antigen-enzyme-labeled antibody After thorough washing, the substrate is added with TMB for color development. TMB is converted into blue under the catalysis of HRP enzyme, and Under the action of transforming into the final yellow. The color depth is positively correlated with interleukin-8 (IL-8) in the sample. Enzyme label The instrument measures the absorbance (OD value) at a wavelength of 450 nm, and calculates the porcine interleukin 8 (IL-8) in the sample through the standard curve concentration. 1 30 times concentrated washing solution 20ml × 1 bottle 7 stop solution 6ml × 1 bottle 2 Enzyme label reagent 6ml × 1 bottle 8 standard (960ng / L) 0.5ml × 1 bottle 3 Enzyme label coated plate 12 wells × 8 strips 9 standard dilutions 1.5ml × 1 bottle 4 Sample diluent 6ml × 1 bottle 10 instructions 1 copy 5 Developer A solution 6ml × 1 bottle 11 2 sealing film 6 Developer B liquid 6ml × 1 / bottle 12 1 sealed bag 1. The ELISA kit should be equilibrated at room temperature for 15-30 minutes before being taken out from the refrigerated environment. After use, the slats should be stored in sealed bags. 2. Crystals may be precipitated in the concentrated washing liquid, which can be heated and dissolved in the water bath during dilution, and the results will not be affected during washing. 3. The sample adder should be used in each step of sample addition, and its accuracy should be regularly checked to avoid test errors. One sample loading time is best Control within 5 minutes, if the number of specimens is large, it is recommended to use a volley gun to add samples. 4. Please make the standard curve at the same time of each measurement, it is better to make the complex hole. If the content of the test substance in the specimen is too high (sample OD value Is greater than the OD value of the first well of the standard product), please dilute it with a certain multiple (n times) of the sample diluent before measuring. When calculating, please multiply the total dilution factor (× n × 5). 5. The sealing film is limited to one-time use to avoid cross contamination. 6. Please keep the substrate away from light. 7. Strictly follow the instructions, and the test results must be determined based on the reading of the microplate reader. 8. All samples, washing liquid and various wastes should be treated as infectious agents. 9. The components of different batches of this reagent shall not be mixed. 10. If it is different from the English manual, the English manual shall prevail. Storage conditions and validity period 1. Kit storage :; 2-8 ℃. 2. Validity: 6 months ELISA kit We could make different size of Color Packaging Box and Printing Label base on your design.we aer the China Hang Tag,Printing Label,Cardboard Boxes,Paper Bags Manufacturer and Supplier. Printing Label Printing Label,Price Tag,Color Tag Xing Hua Printing Factory , https://www.xinghuaprint.com
2013 new ELISA kit operating instructions