Root ion velocity is related to aluminum toxicity tolerance

In acid soil, aluminum (Al) affects the growth of plant roots, and acid (low pH) also affects the growth of plant roots, and the root aluminum toxicity is consistent with acid (low pH) toxicity. This raises a question for researchers: Is the plant's tolerance to aluminum toxicity and acid resistance controlled by the same mechanism? To clarify this issue, Australian researchers selected four genotypes of Arabidopsis (wild-type, aluminum-tolerant mutant arr104, aluminum-tolerant mutants als3 and als5) as research materials for acid stress and acid / aluminum After combined poisoning stress, the H + and K + flow rates of the roots were measured by non-damage micro-measurement techniques. At the same time, data indicators such as alkalization ability around the roots, internal potassium ion concentration, and plasma membrane potential change (Em) were also measured. The results showed that both als5 and alr104 mutants were resistant to acidic stress, and alr104 was also resistant to the combined acidic / aluminum stress. The aluminum toxicity sensitivity of als5 and als3 were basically the same. Aluminum-induced aluminum toxicity-sensitive mutants (als3 and als5) have stronger H + influx in root elongation and H + outflow in mature regions than wild-type and arr104. The change trend of membrane potential (Em) in the root extension zone is consistent with the change of K + flow: arr104 and wild-type Arabidopsis thaliana root extension zone K + outflow is stronger than aluminum-sensitive type. Finally, the researchers concluded that the aluminum toxicity tolerance of arr104 is related to membrane potential depolarization, strong K + outflow, and strong H + inflow. Strong H + inflow leads to alkalization of the soil around the root system, thereby alleviating the combined stress of acidity and aluminum toxicity. ; The acid resistance of als5 is mainly related to the efficient absorption of H +, and this absorption can be terminated by Al stress. The above research results provide strong experimental evidence for the mechanism of aluminum toxicity, and also lay a theoretical foundation for the development of soil aluminum toxicity control programs. Keywords: Aluminium toxicity, H + flux, K + flux, plasma membrane potential Reference: J. Bose et al. J. Exp. Bot, 2010, 61 (11): 3163-3175

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