Enzyme-labeled antigen convection immunoelectrophoresis

Enzyme-labeled antigen convection immunoelectrophoresis technology remembers the principle of antigen convection immunoelectrophoresis. In enzyme-labeled antigen convection immunoelectrophoresis, enzyme-labeled antigen replaces unlabeled antigen. Under alkaline conditions, the enzyme-labeled antigen in the gel moves to the positive electrode, and the antibody is The osmotic fluid flow slowly moves to the negative electrode, and then forms an enzyme-labeled antigen-antibody immune complex, and finally develops color with the substrate. Enzyme-labeled antigen convection immunoelectrophoresis program (detection of schistosomiasis) antigen purification of schistosomiasis egg protein ; Health, sick rabbit serum or health, patient serum; Substrate solution: first prepared into a saturated benzidine solution, and then diluted with an equal amount of 0.01mol / L pH 7.6 boric acid buffer, add H2O2 before use to make H2O2 final concentration 0.01%. (3) Experimental procedure: Convection immunoelectrophoresis and rinsing are performed according to conventional methods. In the substrate and hydrogen donor solution, room temperature (20-25 ℃) reaction 3h. Observe and record. Discuss the storage time of enzyme-labeled egg antigen for home within 1 month. Enzyme-labeled antibodies are prepared, and the corresponding antigen can also be detected by this method.

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