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Porcine Rotavirus Antigen (RV-Ag) ELISA Kit Instructions
Operation step 1. Numbering: number the corresponding microwells in sequence, each plate should be set with 2 wells for negative control, 2 wells for positive control, and 1 well for blank control (the blank control well does not add samples and enzyme reagents, the rest of the operations are the same ) 2. Sample addition: Add 50 μl of negative control and positive control to the negative and positive control wells respectively. Then add 40μl of sample diluent to the sample well, and then add 10μl of the sample to be tested. Add the sample and add the sample to the bottom of the well of the microplate, try not to touch the wall of the well, gently shake to mix, 3. Incubation: seal the plate with the sealing film and incubate at 37 ℃ for 30 minutes. 4. Mixing solution: Dilute 30 times concentrated washing solution with distilled water 30 times before use. 5. Washing: Carefully remove the sealing film, discard the liquid, spin dry, fill each well with washing solution, let it stand for 30 seconds and then discard , Repeat this 5 times, pat dry. 6. Add enzyme: add 50μl of enzyme label reagent to each well, except blank well. 7. Incubation: The operation is the same as 3.