The difference between the tunnel kit and the ELISA kit: the tunnel kit is a deoxyribonucleotide terminal transferase-mediated nick end labeling method. Principle: In apoptosis, chromosomal DNA double-strand breaks or single-strand breaks produce a large number of sticky 3' ends, which can deoxyribonucleotides and fluorescence under the action of deoxyribonucleotide terminal transferase (TdT). A derivative formed by a hormone, a peroxidase, an alkaline phosphatase or biotin is labeled to the 3' end of the DNA, thereby enabling detection of apoptotic cells. Since normal or growing cells have almost no DNA breaks, there is no 3' end formation and few can be stained. TUNEL is actually a combination of molecular biology and morphology. In situ staining of intact single apoptotic nuclei or apoptotic bodies can accurately reflect the typical biochemical and morphological features of apoptosis. Paraffin-embedded tissue sections, frozen tissue sections, cultured cells, and cells isolated from tissues were assayed for cell morphology, and a very small number of apoptotic cells were detected, and thus were widely used in the study of apoptosis. The ELISA test is a highly sensitive, specific, and reproducible experimental diagnostic method. Because of its stable reagents, easy storage, easy operation, and objective judgment, it has been widely used in various fields of immunology. The ELISA test kit is an ELISA for detecting human anti-human hepatitis E (HEV) virus IgM antibody in human serum or plasma in vitro. The ELISA kit uses a qualitative sandwich immunoassay technique to coat microplate plates with synthetic HEV polypeptide antigens. These peptides are highly antigenic peptides in the core amino acid sequence of Chinese type HEV strains, which are derived from the strain. Open reading box 2 and open reading box 3. Samples or standards are added to the wells and incubated, and if HEVIgM antibodies are present, these antibodies bind to the polypeptide antigen of the HEV and are immobilized thereon, washing the plate to remove other non-specific antibodies and other components in the sample. Then, the goat anti-human IgM-HRP (horseradish peroxidase) enzyme conjugate was added. After the second incubation, the enzyme conjugate was combined with the HEVIgM antibody bound to the first incubation, and the plate was removed to remove unbound. Enzyme conjugate, TMB substrate solution, enzyme-substrate reaction occurs in the third incubation, only those pores containing the complex formed by HEVIgM antibody and enzyme conjugate will change color, adding sulfuric acid solution The reaction between the enzyme and the substrate was terminated, and the OD value was measured at a wavelength of 450 nm. According to the test standard of the HEVIgM antibody elisa kit, a sample having an OD value greater than or equal to the Cut-Off value was considered to be a preliminary test positive.
baby
safety chair
Baby
safety chair is with whisper, when baby sit down, seat cushion would whisper,
get baby interest in sitting. Products are used of new PP material, can bear
80kgs weight, very safe for baby in any age. Suit for baby bathing, washing,
even playing, in any place need sit.
Pack
6pcs in one carton. OEM is possible, customized colors are also available. Let
we know your demand, suitable recommendation would be provided within 2 days.
Quality
is our culture, service is also what we emphasis. Choose us, you just give
order, we will do the following all things, keep you posted on order details
periodically, and delivery on time.
Baby Safety Chair,Baby Plastic Chair,Baby Table Chair,Baby Booster Seat ​Taizhou Howawa Baby Products Co., Ltd. , https://www.howawa.com
The difference between the tunnel kit and the ELISA kit