Detection Range: 96T1pg/ml - 32pg/ml
Purpose of Use: This kit is designed to quantify the level of interferon-beta (IFN-β) in human serum, plasma, and other related biological samples.
Principle of the Assay: The kit employs a double-antibody sandwich ELISA method to detect IFN-β in the sample. A microtiter plate is pre-coated with a purified monoclonal antibody specific to human IFN-β, forming a solid-phase capture antibody. After adding the sample, IFN-β binds to the immobilized antibody. A horseradish peroxidase (HRP)-labeled secondary antibody is then introduced, which recognizes and binds to the captured IFN-β, forming a complex of antibody-antigen-enzyme-labeled antibody. Following a thorough wash to remove unbound components, the substrate TMB (3,3',5,5'-tetramethylbenzidine) is added. Under the catalytic action of HRP, TMB turns blue and then changes to yellow upon the addition of an acidic stop solution. The intensity of the color is directly proportional to the concentration of IFN-β in the sample. The optical density (OD) is measured at 450 nm using a microplate reader, and the IFN-β concentration in the sample is determined by comparing the OD value to a standard curve generated from known concentrations of IFN-β.
This assay offers high specificity and sensitivity, making it suitable for research and clinical applications where accurate quantification of IFN-β is required. The kit provides reliable results when used according to the manufacturer's instructions.
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