Blood collection involves several methods, including capillary and venous techniques. Capillary blood is typically collected from the edge of the earlobe or the side of the ring finger. For venous sampling, a needle is inserted into a vein, usually in the arm. It is important to discard the first drop of blood to avoid contamination and then collect a small drop, approximately 3 mm in diameter, on one end of the slide while keeping it horizontal. Slide preparation is crucial for accurate results. New slides are recommended, or they can be cleaned by boiling in a washing powder solution for 2 minutes, followed by rinsing with running water. Soaking them in a cleaning solution for about an hour or overnight, then rinsing thoroughly, and finally soaking in 95% alcohol for an hour helps ensure cleanliness. After drying, the slide should be wiped with a clean cloth or used as a sterile disposable slide. Coverslips should also be new. The push slide is another clean slide with a flat edge, or a cover slip can be used directly. To create a smear, hold the push slide at a 30-degree angle relative to the blood slide and gently move it back slightly before contacting the blood drop. The blood will spread along the edge of the push slide, and the slide should be moved smoothly at a consistent speed until the entire blood film is spread evenly. The quality of the smear should be uniform, with proper thickness, and clear head, tail, and edges. A large blood drop, fast movement, or a larger angle can result in a thicker smear. Before staining, the blood film must be completely dry to prevent it from peeling off during the process. Use a crayon to mark both ends of the smear and place it on a staining rack. Staining time depends on the dye concentration, room temperature, and cell count. A lighter dye may require longer staining, while the dye solution should not be too low to avoid evaporation. Do not pour out the dye before rinsing; instead, rinse with running water carefully. If the stain is too light, counterstaining can be done. If it’s too dark, it can be washed with water or decolorized using methanol. For microscopic examination, turn on the microscope and adjust the illumination. Place the stained slide on the stage and begin with a 10× objective lens, adjusting the coarse and fine focus to get a clear image. Switch to the desired objective lens and adjust the focus accordingly. When using an oil immersion lens, apply cedar oil to the sample area and adjust the focus again. Record your observations, and after finishing, wipe the lens with xylene-soaked lens paper. Finally, turn off the power. New products include: bs-4725R specific cyst disease liquid protein 15 | GCDFP15, bs-4726R specific cyst disease liquid protein 15 | GCDFP15, bs-11794R growth hormone releasing factor receptor antibody | GHRHR, bs-12026R G protein coupled receptor 115 antibody | GPR115, bs-11803R G protein coupled receptor 10 antibody | GPR10, and many more. These reagents are essential for various immunohistochemistry and molecular biology applications.

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